A three day course for those in industry and academia who wish to gain a theoretical and practical understanding of the exciting possibilities and developments within the field of proteomics.
Proteomics requires the identification and quantitation of ever-smaller amounts of proteins in increasingly complex systems. Modern approaches have been established to enable this to be accomplished in an automated, sensitive and selective fashion. This course will provide the theoretical underpinning of the methods used, with ten practical sessions undertaken in a fully equipped, state-of-the-art laboratory and include sample preparation strategies, ESI, ESI-MS/MS, nanoflow LC, data dependent/independent acquisition, database searching, multiple reaction monitoring and neutral loss scans. Regular discussion groups help to reinforce theory/practical sessions and serve as a forum for exchange of ideas. Quantitative case studies from biomarker discovery to prokaryotic and eukaryotic systems will be presented.
The course will be informal but intensive with numbers limited to 15 and a high level of staffing to encourage interaction, questions and discussion. Participants will have the opportunity to discuss their interests and research with course tutors and other participants.
The lecture programme has been designed to provide the underpinning theory required in modern proteomics studies. This will include protein separation, mass spectrometric characterisation, quantification, sequencing and the use of databases for identification of proteins. This will be illustrated by the use of appropriate practical examples. See also the practical programme below, that accompany the lectures and are also listed by day order.
- ESI/MALDI ionisation
- Mass analysers
- Mass accuracy and resolution
- Intact protein molecular weight determination
- Protein separation approaches
- Digestion strategies
- MS/MS of peptides
- Interpretation of MS/MS spectra
- Database creation and searching
- Labelled and non-labelled approaches to differential proteomics
- Characterisation of post-translational modifications
- Case studies
- Recent developments
Accompanying the lecture programme is an intensive practical programme concentrating on the skills required to carry out modern proteomic studies.
- Protein molecular weight determination
- Sample preparation and tryptic digestion
- Analysis of digested proteins by means of ESI-MS and ESI-MS/MS
- Nano liquid chromatography
- Data dependent acquisition and database searching
- Interpretation of MS/MS data
- Multiple reaction monitoring and absolute quantitation
- Offline database interrogation using MS/MS data
- Data independent acquisition
- Neutral loss scan
- Labelled and non-labelled mass spectrometry-based quantitative proteomics
How to register
Print page two of the Proteomics leaflet (available in the downloads section above) to aquire the application form, to be completed and posted to the address provided, or contact Charlotte Moonan (see external contact below). The leaflet also has further details about the course and registration fee required. Further information is available form the Warwick University website (see external links).