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Aphids mount multiple defence strategies to resist insecticides

Visit  Rothamsted Research website

28 June 2010

The peach potato aphid, Myzus persicae is the most important aphid pest of crops in many regions of the world due to its large host range and the damage it causes through direct feeding and its ability to transmit more than 100 plant viruses. This species has developed resistance to most insecticides although a class of compounds called the neonicotinoids have remained an effective and popular control measure. However, recent cases of neonicotinoid resistance in M. persicae represent a threat to the long-term effectiveness of these insecticides as well.

Work on other insect species has shown that resistance can arise through increased production of metabolic enzymes called cytochrome P450s that detoxify the insecticide. A new study of neonicotinoid resistance in M. persicae, published in PLOS genetics by scientists at Rothamsted Research, an institute of BBSRC, and collaborators at University College London, is the first to find resistance associated with an increase in the number of copies of a P450 gene in a major agricultural pest.

The study made use of recent advances in genomics to investigate all genes encoding detoxification enzymes in a resistant strain of M. persicae. Dr Chris Bass, who led the genomics research said, 'This study builds on the work of Graham Moores and Despina Philippou at Rothamsted, who provided strong evidence of a role for P450-mediated detoxification in resistance*. Now we have demonstrated that resistance is associated with multiple duplications of a single P450 gene, with the resistant aphids carrying ~18 copies of the gene compared to the normal 2 copies found in susceptible aphids.' This may enable aphids to rapidly breakdown insecticides and so avoid death.

Genomic analyses also revealed that several genes encoding proteins that make up the insect cuticle are over-expressed in resistant aphids. The paper's lead author, Dr Mirel Puinean said, 'This suggests that an increase in the production of cuticular proteins in resistant aphids could reduce the amount of neonicotinoids that penetrate the insect's body and our biochemical assays supported this.' However, further work is needed to discover exactly how the increased production of cuticular proteins boosts the body armour of aphids that are resistant to insecticides.

Levels of neonicotinoid resistance currently being reported are insufficient to impair the field effectiveness of these insecticides. However, this work highlights the remarkable capacity of aphids to adapt and the need for continued research and informed management strategies to prevent resistance becoming more potent and widespread.

ENDS

Notes to editors

This paper is published in PLOS genetics as "Amplification of a cytochrome P450 gene is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae" by Alin M. Puinean, Stephen P. Foster, Linda Oilphant, Ian Denholm, Linda M. Field, Neil S. Millar, Martin S. Williamson and Chris Bass (Please see http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000999). For more information see http://www.rothamsted.ac.uk/bch/PersonalWebpage/ChrisBass.html

This work was funded as part of an Institute Career Path Fellowship award to Dr Chris Bass by BBSRC and a BBSRC Industrial Partnership Award to Dr Ian Denholm, Martin Williamson and Professor Lin Field at Rothamsted Research and Professor Neil Millar at UCL.

*See also: Philippou D, Field LM, Moores GD (2009) Metabolic enzyme(s) confer imidacloprid resistance in a clone of Myzus persicae (Sulzer) (Hemiptera: Aphididae) from Greece.

Pest Manag Sci 66: 390-395. (http://www3.interscience.wiley.com/journal/123193309/abstract)

Guidelines on managing resistance in M. Persicae have been published by the Insecticide Resistance Action Group (IRAG). Please see: http://www.pesticides.gov.uk/uploadedfiles/Web_Assets/RAGs/GuidelinesOnInsecticideResistanceInMyzusPersicae_2005.pdf

About Rothamsted Research

Rothamsted Research is based in Hertfordshire and is one of the largest agricultural research institutes in the country. The mission of Rothamsted Research is to be recognised internationally as a primary source of first-class scientific research and new knowledge that addresses stakeholder requirements for innovative policies, products and practices to enhance the economic, environmental and societal value of agricultural land. The Applied Crop Science department is based at Broom's Barn, Higham, Bury St. Edmunds. North Wyke Research is located near Okehampton in Devon. Rothamsted Research is an institute of the Biotechnology and Biological Sciences Research Council.

For further information, please contact the Rothamsted Research Press Office. Dr Sharon Hall (Tel: 01582 763 133 ext 2757 or email sharon.hall@rothamsted.ac.uk) or Dr Adélia de Paula (Tel: 01582 763 133 ext 2260 or email adelia.depaula@rothamsted.ac.uk).

About UCL

Founded in 1826, UCL was the first English university established after Oxford and Cambridge, the first to admit students regardless of race, class, religion or gender, and the first to provide systematic teaching of law, architecture and medicine. UCL is the fourth-ranked university in the 2009 THES-QS World University Rankings. UCL alumni include Marie Stopes, Jonathan Dimbleby, Lord Woolf, Alexander Graham Bell, and members of the band Coldplay. UCL currently has over 12,000 undergraduate and 8,000 postgraduate students. Its annual income is over £600 million.

About BBSRC

BBSRC is the UK funding agency for research in the life sciences. Sponsored by Government, BBSRC annually invests around £470M in a wide range of research that makes a significant contribution to the quality of life in the UK and beyond and supports a number of important industrial stakeholders, including the agriculture, food, chemical, healthcare and pharmaceutical sectors.

BBSRC provides institute strategic research grants to the following:

  • The Babraham Institute
  • Institute for Animal Health
  • Institute for Biological, Environmental and Rural Studies (Aberystwyth University)
  • Institute of Food Research
  • John Innes Centre
  • The Genome Analysis Centre
  • The Roslin Institute (University of Edinburgh)
  • Rothamsted Research

The Institutes conduct long-term, mission-oriented research using specialist facilities. They have strong interactions with industry, Government departments and other end-users of their research.