Related links

Share this page:
Other services (opens in new window)
Sets a cookie

Genome sequence sheds new light on how plants evolved nitrogen-fixing symbioses

Visit John Innes Centre websiteVisit The Genome Analysis Centre website

17 November 2011

The genome of Medicago, a close relative of alfalfa and a long-established model for the study of legume biology, has been sequenced by an international team of scientists, capturing around 94 per cent of its genes.

The research, funded by BBSRC amongst others, gives new insights into the evolution of the Papilionoid subfamily of legumes, which includes peas, soybean and all legumes grown as crops.

Genome sequence sheds new light on how plants evolved nitrogen-fixing symbioses - 17 November 2011Image showing Medicago. Image: John Innes Centre

Medicago. Image: John Innes Centre

The scientists hope that studying Medicago might eventually help them to produce varieties of important food crops that can fix their own nitrogen. If they could achieve this it would help reduce our reliance on fertilisers which would improve the sustainability of crop production and contribute to ensuring food security.

Plants in this family can house and work with bacteria to provide them with nitrogen from the air. The new findings suggest this useful trait can be partly attributed to a genetic event 58 million years ago, when duplicate genes of the whole genome were created.

The duplication of genes allows new mutations and functions to occur, while maintaining the roles of original genes.

"The details of the genome shed new light on Medicago, the plant model that will help unlock the workings of nitrogen fixation we hope within our lifetime," said Professor Giles Oldroyd from the John Innes Centre on Norwich Research Park.

"A whole genome duplication appears to have played a crucial role in the dramatic radiation of papilionoid legumes," said director of the The Genome Analysis Centre Professor Jane Rogers, also based on Norwich Research Park.

"This shaped their genomes and contributed to their success, enhancing their value to humans."

One outcome was the emergence of additional genes that went on to become specialised for functions related to the root nodule. This organ is formed by legumes to house symbiotic nitrogen-fixing rhizobial bacteria, which do the job of providing their hosts with a form nitrogen they can use while the host plant provides the bacteria with sugars and proteins.

The new findings indicate that some important components for nodulation might have evolved from ancient genes and were then duplicated and added to 58 Mya. The capacity for interaction with symbiotic bacteria and fungi is derived from ancient machinery that was added to following the whole genome duplication, leading to the complex relationship that benefits Medicago and other legumes today.

"Legume symbiosis with rhizobia is the largest source of natural, non-synthetic, nitrogen fertilizer in agriculture," said Professor Nevin Young from the University of Minnesota.

"We sequenced the Medicago genome primarily to learn about its evolution."

The scientists also found in the Medicago genome more NBS-LRR genes, a class of resistance genes, than in any other plant genome to date.

"This is potentially a useful resource to exploit," said Professor Oldroyd.

The John Innes Centre and The Genome Analysis Centre both receive strategic funding from BBSRC.


Notes to editors

Reference: The Medicago Genome Provides Insight into the Evolution of Rhizobial Symbioses

The work was funded by the EU Framework Program 6 (FP6), L'Agence Nationale de la Recerche de la France, US National Science Foundation, and the Noble Foundation. The UK-based members received funding from the Biotechnology and Biological Sciences Research Council (BBSRC).

The lead sequencing groups:

  • University of Oklahoma
  • J. Craig Venter Institute
  • Genoscope
  • Wellcome Trust Sanger Institute

Other lead institutions:

  • University of Minnesota
  • CNRS/INRA-Toulouse
  • John Innes Centre
  • Noble Foundation
  • University of Wageningen
  • MIPS
  • Ghent University
  • National Center for Genome Resources (NCGR)

Altogether, there are 128 co-authors at 31 institutions in 8 countries.

About the John Innes Centre

The John Innes Centre,, is a world-leading research centre based on the Norwich Research Park The JIC's mission is to generate knowledge of plants and microbes through innovative research, to train scientists for the future, and to apply its knowledge to benefit agriculture, human health and well-being, and the environment. JIC delivers world class bioscience outcomes leading to wealth and job creation, and generating high returns for the UK economy. JIC is one of eight institutes that receive strategic funding from the Biotechnology and Biological Sciences Research Council and received a total of £28.4M investment in 2010-11.

About The Genome Analysis Centre

TGAC,, is a centre for the application of genomics and bioinformatics to key strategic areas of biological science and a specialist in next generation sequencing. It is located on the Norwich Research Park. It was established in 2009 by the Biotechnology and Biological Sciences Research Council, in partnership with the East of England Development Agency, Norfolk County Council, South Norfolk Council, Norwich City Council and the Greater Norwich Development Partnership. TGAC is one of eight institutes that receive strategic funding from the BBSRC, with a total of £5.4M investment in 2010-11.


BBSRC invests in world-class bioscience research and training on behalf of the UK public. Our aim is to further scientific knowledge, to promote economic growth, wealth and job creation and to improve quality of life in the UK and beyond.

Funded by Government, and with an annual budget of around £445M, we support research and training in universities and strategically funded institutes. BBSRC research and the people we fund are helping society to meet major challenges, including food security, green energy and healthier, longer lives. Our investments underpin important UK economic sectors, such as farming, food, industrial biotechnology and pharmaceuticals.

For more information about BBSRC, our science and our impact see: .
For more information about BBSRC strategically funded institutes see: .

External contact

Zoe Dunford, JIC/TGAC Press Office

tel: 01603 255111

Andrew Chapple, JIC/TGAC Press Office

tel: 01603 251490